Microtuberization of potato (Solanum tuberosum L.)

  • D-r. Liljana Koleva Gudeva
  • D-r. Fidanka Trajkova


This paper presents the results of the examination of the impact of phytohormon gibberellic acid GA3 on sprouts formation in in vivo conditions, and the impact of the phytohormones on induction of microtuberization under in vitro conditions in several varieties of seed tubers and mercantile potato tubers (Solanum tuberosum L.) Seed tubers potatoes tests were made with varieties Dido, Marabel, Agria, Ambition, and Agriko, while Agria SR, Agria BE and Andrea were the mercantile varieties of potato used in the experiment.

Experiments in vitro conditions were set by two types of explants: sprouts and nodal explants on the MS (Murashige & Skoog) medium with the addition of several different combinations and concentrations of cytokinines and auxins. Microtubarization was stimulated by increasing the percentage of sugar in MS medium from 30 g/l sucrose, to 40, 60 and 90 g/l sucrose.

The treatment with 22 ppm GA3 was most effective for both types of potatoes seed tubers and mercantile potato tuber. In all tubers treated with gibberellic acid treatment resulted in de novo sprouting of tubers.

On the MS + 6 mg/l BAP + 2 mg/l NAA + 90 g/l С12Н22О11 microtuberization reached up to 92.85% for the variety Agria BC in nodal culture of potato (Solanum tuberosum L.).