Content of Photosynthetic Pigments in Pepper In Vitro Cultures
Abstract
Chlorophylls are some of the most important chelates in nature. Theyare capable of channelling the energy of sunlight into potential chemical energythrough the process of photosynthesis. Photosynthesis is complex biologicalprocess which occurs in in vitro conditions too, but in alternative pathways onbiosynthesis of photosynthetic pigments.The purpose of our examinations was to evaluate the content ofphotosynthetic pigments chlorophyll a, b and a+b in the pepper cultureCapsicum annuum L. of the varieties Kurtovska Kapija and Golden Medal onthe MS (Murashige and Skoog) medium, especially in shoot culture, culture ofcallus from meristem and callus, and as well as cotyledon culture. All peppertissue cultures were subject of investigation regarding the effect of plant grownregulators on in vitro synthesis of photosynthetic pigments.References
Belyaeva, B.O. 2003. Studies of chlorophill biosintesis in Russia. Photosintesis Research 76: 405-411.
Culafic, Lj., Cerovic, Z., Naunovic, G., Konjevic, R., 2000. Praktikum Fiziologija Biljaka, NNK-International, Beograd.
Murashige, T. (1979): Principles of Rapid Propagation. Propagation of Higer Plants Through Tissue Culture. pp 14-24.
Murashige, T. and Skoog, F. (1962): A revised medium for rapid growth and bio assay with tobacco tissue cultures, Phisiologya Planatarum, Vol. 15: 473-497.
Neskovic, M., Konjevic R., Culafic, LJ., 2003. Fiziologija Biljaka, NNK-International, Beograd.
Sasson, A. 1991. Production of useful biochemicals by higer-plant cell culture: biotechnologycal and economic aspects. CIHEAM – Options Mediterraneennes. 14: 59-74.
Ticha, I., Cap, F., Pacovska, D., Haisel, D., Capkova, V. and Schafer C. 1998. Culture on sugar medium enhances phptosintetic and high light resistance of plantlets grown in vitro. Physiologia Planatarum Vol. 102: 155- 162.
