Obtaining Haploids in Anther Culture of Pepper Capsicum annuum L. and Their Inclusion in the Breeding Process

Abstract

The frequency of obtained androgenic plants depends highly on thegenotype; therefore the low rate of haploid recovery limits the utility of antherculture in pepper breeding.The aim of this study was establishment of effective in vitro technologyfor study of haploid and diploid plant regenerants; induction of embryogenesisin pepper anther culture; development of the embryos into regenerants aswell as successful adaptation and acclimatization of regenerants from sterileto greenhouse conditions. In the present study, the effectiveness of inducedandrogenesis in anther culture of several Bulgarian and Macedonian peppergenotypes was investigated.The collected seed material is excellent possibility for further breedingprocesses, cytogenetic and other molecular level research.The results of this paper derived from international bilateral Macedonian-Bulgarian Joint Research Project: “Obtaining haploids in anther culture ofpepper Capsicum annuum L. and their inclusion in the breeding process”,managed by the fi rst two authors and with participation of the coauthors.