The Content of some Biogene Elements and other Physiological Characteristics of Pepper (Capsicum annuum L.) obtained In Vivo and In Vitro Conditions
AbstractApical buds were isolated from aseptically germinated seeds of pepper (Capsicum annuum L.), then they were cultivated on MS medium (Murashige and Skoog) with different concentrations and combinations of hormones. After four weeks, pepper shoots were obtained from apical buds in culture tissue in vitro conditions. After eight weeks the plantlets were transferred from sterile (laboratory) conditions in the laboratory to fi eld conditions where the regenerates became adapted to the environment. Growth regulators affect the translocation of mineral substances in the vegetative organs of plant species. The aim of this research was to determine the content of certain biogenic elements and some photosynthesis pigments in pepper produced in vivo and in vitro conditions. The results showed that the plants obtained of conventional production from in vivo conditions, which were used as a control, compared with in vitro obtained plants expressed signifi cant differences in only a few of the examined features. Differences occurred primarily due to different hormonal treatment in the stages of seedling preparation of the seedling. In vitro obtained plants have tended to preserve the juvenility characteristics i.e. rejuvenating, which characteristic is proved with the obtained results from the examined parameters.
Arous, S., Boussaid, M., Marrach, M. 2001: Plant regeneration from zygote hypocotyls embryos of Tunisian chilly Capsicum annuum L. J. Appl. Hort. 3(1): 17-22.
Dabuaza, M. and Pena L., 2001: High effi ciency organogenesis in sweet pepper Capsicum annuum L. tissues from different seedlings explants. Plant Growth regulations 33: 221-229.
Fari, M., Czako, M. 1981: Relationship between position and morphogenetic response of pepper hypocotyls explants cultured in vitro, Scientia Horticulturae, 5:205-213.
Ficher, M. 1990: Establishment of (Pepper nigrum) in vitro, Acta Horticulturae, 275: 285 - 291.
Joshy, A. Kothari S.L. 2007: Height copper levels in the medium improved shoot bud differentiation and elongation from the cultured cotyledons of Capsicum annuum L. Plant Cell Tissue and Organ Culture 88: 127-133.
Garcia, R. A.1990: Tissue and cell culture of pepper (Capsicum annuum L. c.v. Pico and Piqulio), APHF/SECH, Jun 1990, p. 249 - 254.
Gunai, L. and Rao, P.S. 1978: In vitro plant regeneration from hypocotyls and cotyledon explants of red pepper (Capsicum), Plant Science Letters, 11: 365 - 372.
Hosain, A., Konisho, K., Minami, M., Nemoto K. 2003: Somaclonal variation of regenerated plants in chili pepper Capsicum annuum L. Euphitica 130: 233-239.
Husain, S., Jain A., Kothari S.I. 1999: Phenolatic acid improves bud elongation and in vitro plant regeneration effi cienty of Capsicum annuum L. Plant Cell Reports 19:64-68.
Колева-Гудева Л. и Спасеноски М. (2001): Ефектот на некои цитокинини врз органогенезата на пиперката (Capsicum annuum L.). Годишен зборник на Институт за јужни земјоделски култури, Струмица. Вол 1: 31-35.
Колева-Гудева Л., Митрев С. и Спасеноски М. (2001): Можности за примена на некои нови методи за производство на безвирусен посадочен материјал. Годишен зборник на Институт за јужни земјоделски култури, Струмица. Вол 1: 37-45.
Kisaburo, H., Zhiqing, Y., Kenij, K. 1988: The effect of cotyledon explants and culture condition on in vitro formation of advention buds in red pepper (Capsicum annuum L.) Agricultural Science, 37: 153 - 159.
Mathewa, H and Rao, P.S., 1984: In vitro response of black pepper (Pepper nigrum), Current Science, Vol. 53, No 4: 183 -185.
Murashige, T. and Skoog, F. 1962: A revised medium for rapid grouth and bio assay with tobacco tissue cultures, Phisiologia Planatarum. Vol 15: 473-497.
Sim, S.L.1986: Propagation of single - node pepper Piper nigrum auttings, Journal Announcment 1037: 105 - 113.
Spasenoski, M. 1993: Vegetative propagation of some plant secies in vitro and possibilities for obtaining health plant matriali. Proceedings of XVIII Plant protection meeting, Ohrid R. of Macedonia, 1993, Vol. 5: 145 - 148.
Swart, H.J. 1993: Genetic and epigenetic effects and related problems, Post culture behavior, p. 95 - 121.