• Irena Petrova
  • Liljana Koleva Gudeva


In this paper the results of influence of phytohormone gibberellic acid GA3 on sprout formation in in vivo conditions and in vitro microtuberization of the potato varieties Agria, Agata, Sunshine, Ultra and Marabel are presented. The tubers from all varieties utilized in the experiment were certified potato seed tuber material.

The experiment in in vitro conditions was established on  sprout explants and nodal segment explants on the MS medium (Murashige & Skoog, 1962) with addition of different combination and concentration of auxins and cytokines. Microtuberization was stimulated by rising the concentration of sucrose in MS medium from 40g/l to 60 g/l and 80g/l, respectively.

The in vivo tuber treatment with 30mg/l GA3 was the most effective treatment for all potato varieties in proliferation of sprouts. All tubers that were treated with GA3 resulted in de novo sprouting of tubers.

The variety Agata resulted with 100% of microtuberization from nodal segment explants on MS medium supplied with 40g/l and 80g/l sucrose. Microtuberization of the variety Sunshine was stimulated with addition of 80 g/l sucrose in MS medium.

The developed microtubers were detached from the nodal segments and subcultured on new MS medium supplied with BAP 4mg/l, KIN 4mg/l and 8% of sucrose to increase their weight.


Abbott, A.J., and Belcher, R., 1986. Potato Tuber Formation in vitro. In: Plant Tissue Culture and Its Agricultural Application. Withers, L.A. and Anderson, P.G. (Eds.). London: Butt Worth: 113- 132.
Akhtar, N., Munawar, H., Hussain, M. and M. Mahmood, M. (2006). Sterile shoot production and direct regeneration from the nodal explants of potato cultivars. Asian Journal of Plant Sciences, 5 (5), 885-889.
Apichai, N., 1988. Microtuber production of potato (Solanum tuberosum L.) in vitro. Journal of the National Research Council of Thailand, 20: 2: 19-40.
Azar, A., Kazemiani, S. and Yarmohamadi, F. (2013). Effect of Sugar/Osmotica Levels on in vitro Microtuberization of Potato (Solanum tuberosum L.). Russian Agricultural Science,39 (2), 112-116
Burlingame, B., Mouillé, B., & Charrondiére, R. (2009). Nutrients, bioactive non-nutrients and antinutrients in potatoes. Journal of Food Composition and Analysis, 22, 494–502.
Camara, M., Vandenberghe, L., Rodriguez, C., Oliveira, J., Faulds, C., Betrand, E.,et al. (2018). Current advances in gibberellic acid (GA3) production, patented technologies, and potential applications. Planta, 248 (5), 1049-1062.
Coleman, K.W., Danielle, J.D. and Colleman, S.E., 2001. Potato microtuber as research tools: A Review. Am J Potato Res.(78): 47-55.
Dhital, S.P., Lim, H.T. & Manandhar, H.K. (2010). Direct and efficient plant regeneration from different explant sources of potato cultivars as influenced by plant growth regulators. Nepal Journal of Science and Technology, 12, 1–6.
Dodds, J.H., Silva-Rodrigez, D. and Tovar, P., 1992. Micropropagation of potato (Solanum tuberosum L.). In: Biotechnology in Agriculture and Forestry: High-Tech and Micropropagation III, (Ed. Bagaj, Y.S.P), Springer,Berlin, Heidelberg, New York, 19: 91-106.
Ferreira, W.M., Suzuki, R.M., Pescador, R., Figueiredo, R. & Kerbauy, G.B. (2011) Propagation, growth, and carbohydrates of Dendrobium Second Love (Orchidaceae) in vitro as affected by sucrose, light and dark. Journal of In Vitro Cellular Development Biology, 47(3), 420–427.
Iarema, L., Cruz, C.F., Saldanha, C.W., Dias, L.C., Vieira, R.F., Oliveira.et al. (2012). Photoautotrophic propagation of Brazilian ginseng [Pfaffia glomerata (Spreng.) Pedersen]. Plant Cell, Tissue and Organ Culture,110(2), 227-238.
Islam, Jahirul, Sun Phil Choi, Obyedul Kalam Azad, Ji Woong Kim, and Young-Seok Lim. (2020). Evaluation of Tuber Yield and Marketable Quality of Newly Developed Thirty-Two Potato Varieties Grown in Three Different Ecological Zones in South Korea" Agriculture 10, no. 8: 327.
Khan, N., Ali, S., Nouroz, F., Erum, S. & Nasim, W. (2018). Effects of sucrose and growth regulators on the microtuberization of CIP potato (Solanum tuberosum L.) germplasm. Pakistan Journal of Botany,50(2), 763-768.
Mohamed, A.H. & Alsadon, A. (2010). Influence of ventilation and sucrose on growth and leaf anatomy of micro propagated potato plantlets. ScientiaHorticulturae Journal,123(3), 295–300.
Murashige, T. & Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiology Plant Journal,15, 473–97.
Rizza, A., Walia, A., Languar, V., Frommer, W. & Jones, A. (2017). In vivo gibberellin gradients visualized in rapidly elongating tissues. Nature Plants, 3(10), 803-813.

Romano, A., Mackie, A., Farina, F., Aponte, M., Sarghini, F., & Masi, P. (2016). Characterisation, in vitro digestibility and expected glycemic index of commercial starches as uncooked ingredients. Journal of Food Science and Technology, 53(12), 4126-4134.
Wang, E.S., Kieu, N.P., Lenman, M. and Andreasson, E. (2020). Tissue Culture and Refreshment Techniques for Improvement of Transformation in Local Tetraploid and Diploid Potato with Late Blight Resistance as an Example" Plants 9, no. 6: 695
Waqas, M.S., Cheema, M.J.M., Hussain, S., Ullah, M.K. and Iqbal, M.M., (2021). Delayed irrigation: An approach to enhance crop water productivity and to investigate its effects on potato yield and growth parameters. Agricultural Water Management, 245, p.106576.

Most read articles by the same author(s)

1 2 > >>