Idetification of the Causer of Tomato Pith Necrosis in the Republic of Macedonia
AbstractIn the period from 2005 to 2009 the occurrence of tomato pith necrosis isinvestigated in the Strumica region. A total number of 24 isolates are investigatedin in vitro conditions and for the fi rst time in the Republic of Macedoniathe presence of the plant pathogen bacteria Pseudomonas mediterranea (Cattaraet al., 2002), causer of tomato pith necrosis, is identifi ed. Physiologicalinvestigations show that the colonies of Pseudomonas mediterranea have corrugatedsurface and produce blue-green pigment. Isolates investigated in in vitroconditions show hypersensitive reaction on tobacco leaves, and pathogenicchanges during artifi cial inoculation of tomato and pepper plants. Biochemicalcharacteristics show that the isolates are Gram negative, produce levan andare positive for catalase, urease and oxidase. All investigated isolates showability to dehydrolize arginine but they do nоt have pectolitic activity, do nоtfl uoresce on medium King B, and do not produce indol, H2S or hydrolizeesculine. Other investigated characteristics show that the isolates can reducenitrates to nitrites, accumulate PHB (Poly-β- hydroksibuturate), grow at 4 and37℃, but not at 41℃. The use of different carbohydrates and acid compoundsis investigated with BIOLOG test. The variety of the bacteria is identifi ed withPCR (Polymerase chain reaction).
Alippi A. M., Ronco B. L., Alippi H.E. (1993): Tomato pith necrosis caused by Pseudomonas corrugata in Argentina. Plant Disease 77: 428.
Alivaziatos, A.S. (1984). Aetiology of tomato pith necrosis in Greece. Proceedings of the Second Working Group on Pseudomonas syringae pathovars. Greece: Sounion, 55-57.
Basim, H., E. Basim., S. Yilmaz, M. Ilkucan (2005): First report of pith necrosis of tomato caused by Pseudomonas mediteranea in Turkey. Plant Pathology 54: 240.
Bradbury, J.F. (1981). Pseudomonas cichorii. C.M.I. Description of Pathogenic fungi and bacteria. No 696, Comm. Mycol. Instit., Kew.
Cattara V., Sutra L., Morineau A., Achouak W., Christen R. and Gardan L (2002): Phenotypic and genomic evidence for the revision of Pseudomonas corrugate and proposal of Pseudomonas mediterranea sp.nov.International Journal of Systematic and Evolutionary Microbiology 52, 1749-1758.
Cattara V., Dawn A., G. Cirvilleri and Vivian A. (2000): Specifi c oligonucleide primers for the rapid identifi cation and detection of the agent of tomato pith necrosis, Pseudomonas corrugata, by PCR amplifi cation: evidence for two diostinct genomic groups. EJPP 106: 753-762.
Cattara V., L. Gardan and M.M. Lopez (1997): Phenotypic heterogeneity of Pseudomonas corrugata strains from southern Italy. Journal of Applied Microbiology 83, 576-586.
Dhanvantari, B.N. (1990). Stem necrosis of greenhouse tomato caused by a novel Pseudomonas sp. Plant Disease, 74 (2): 124-127.
Klement Z, Rudolph K., Sands D.C. (1990): Methods in Phytobacteriology. Budapest, Hungary: Akademiai Kaido.
Lelliot R. A., Billing E., Hayward A.C. (1966): Adeterminative scheme for the fl uorescent plant pathogenic pseudomonads.
Marte, M. (1980): Histological and histochemical observations on tomato stems naturally infected by Corynebacterium michiganense . Phytopathologische Zeitschrift 97, 252 – 271.
Pietro Lo Cantore and Nicola Sante Iacobellis (2002): Necrosi cortiocale e del midollo del pomodore causata da Pseudomonas fl uorescense in Puglia. Informatore Phytopathologico 2002: 54 – 57.
Scarlett, C.M., Fletcher, J.T., Roberts, P. and Lelliott, R.A., (1978). Tomato pith necrosis caused by Pseudomonas corrugata n.sp. Ann. Appl. Biol. 88: 105-114.
Shaad N. W., Jones J.B., Chun W. (2001): Laboratory Guide for Identifi cation of Plant Pathogenic Bacteria, 3rd edn. St Paul, M.N., USA: APS Press, 1-16.
Sutra, L., Siverio, F., Lopez, M.M., Hunault, G., Bollet, C. and Gardan, L. (1997). Taxonomy of Pseudomonas strains isolated from Tomato Pith Necrosis: emended Description of Pseudomonas corrugate and proposal of three unnamed fl uorescent Pseudomonas genomospecies.
Wilkie, J. P., Dye, D.W. (1974). Pseudomonas cichorii causing tomato and celery diseases in New Zealand. N. Z. Journal of Apric.Research, 17, 123- 130.