Races of Xanthomonas Vesicatoria Isolated from Pepper in Macedonia
AbstractFrom 1994 till 1997, few hundreds of strains were isolated from different samples of pepper (Capsicum annuum L.) collected from commercial fields and home gardens throughout Rep. of Macedonia. Bacteria were isolated on semi selective medium from infected plants and identified by a combination of morphological, pathological, biochemical, nutritional and physiological tests. In this study 50 domestic and 3 control strains (races: E-3; 93-1; 71-21 from Florida, USA) were used. All these properties of Macedonian strains were completely same with characteristics of compared control strains, without any significant differences. Additional characterization of strains showed that all were negative for amylolytic activity and ability to utilize dextrin. Those strains belong to type A of X. vesicatoria and none of them belong to type B. Strains of X. vesicatoria were identified to race based on the response to infection of a set of near-isogeneic pepper lines derived from and including Early Californian Wonder (ECW): ECW-10R, ECW-20R and ECW-30R containing the resistance genes bs1, Bs1, Bs2 and Bs3, respectively. The majority of the strains were identified as race P0 - 40%, race P2 - 40%, and the remaining 20% could not be identified to race using these differential pepper lines. All of investigated strains were sensitive to copper sulfate (200Xg/ml) and streptomycin sulfate (100Xg/ml).
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