Characterization of Bacterial Strains of Pseudomonas Syringae pv. Syringae Isolated from Pepper Leaf Spot in Macedonia

  • Sasa Mitrev
  • L Gardan
  • R Samson


A new bacterial leaf spot disease on pepper seedlings (Capsicum annuum cv. ´Kurtovska kapija`) was observed in 1995 in Macedonia. Pseudomonas bacteria were isolated, belonging to LOPAT group Ia. Symptoms similar to natural symptoms were reproduced following inoculation on pepper seedlings. Some isolates produced syringomycin and none of them were pathogenic to lilac. In a numerical taxonomic study of five pepper isolates in comparison with 58 pathovars of P. syringae and 10 related species, the five pepper isolates clustered in one phenon. Considering phenotypic characteristics, serology, DNA relatedness and pathogenicity tests, it was concluded that the pepper strains belong to Pseudomonas syringae pv. syringae.


Arsenijevic M., Balaz J., 1978. (An etiological study of bacterial spot on pepper leaves). Savremena Poljopr. 7-8: 75-86.

Bradbury J.F. 1986. Pseudomonas. In: Bradbury J.F. (ed.). Guide to plant pathogenic bacteria, pp. 110-185. CAB International Mycological Institute, Kew, U.K.

Gardan L., Cotin S., Bollet C., Hunault G., 1991. Phenotypic heterogeneity of Pseudomonas syringae. Van Hall. Research in Microbiology 142, 995-1003.

Gardan L., Shafik H., Belouin S., Broch R., Grimont F., Grimont P.A.D., 1999. DNA relatedness among the pathovars of Psudomonas syringae and description of Pseudomonas tremae sp.nov. and P.cannabina sp.nov. (Sutic and Dowson 1959). International Journal of Systemic Bacteriology 49: 1-10.

Grondeau C., Saunier., Poutier., 1992. Evaluation of physiological and serological profiles of Pseudomonas syringae pv. pisi for pea blight identification. Plant Pathology 41: 495-505.

Gross D.C., De Vay J.E., 1977. Population dynamics and pathogenesis of Pseudomonas syringae in maize and cowpea in relation to the in vitro production of syringomycin. Phytopathology 67: 457-483.

Leliott R. A., Billing E., Hayward A.C., 1966. A determinative scheme for the fluorescent plant pathogenic pseudomonads. Journal of Applied Bacteriology 29: 470-489.

Morton D.J., Ratcliffe T.J., 1964. A recently discovered unidentified foliar disease of pepper seedlings in Georgia. Plant Disease Reporter 48: 89.

Obradovic A., Arsenijevic M., Mijatovic M., Marinkovic N., 1995. Bacterial spot of pepper seedlings. Zastita Bilja, Belgrade 46 (213): 215-220, Beograd.

Person L.H., 1964. A bacterial leaf spot of pepper caused by Pseudomonas syringae. Plant Disease Reporter, 48: 750-753.

Saunier M., Malandrin L., Samson R., 1996. Distribution of Pseudomonas syringae pathovars into twenty-three O Serogroups. Applied and Environmental Microbiology 62: 2360-2374.

Young J.M., 1991. Pathogenicity and identification of the lilac pathogen Pseudomonas syringae pv. syringae van Hall 1902. Annals of Applied Biology 118: 283-298.

Young J.M., Saddler G.S., Takikawa Y., De Boer S.H., Vauterin L., Gardan L., Gvozdyak R.I., Stead D.E., 1996. Names of plant pathogenic bacteria. Review of Plant Pathology 75: 721- 762.


Most read articles by the same author(s)

1 2 3 4 5 > >>