Presence of grapevine leafroll associated virus in the vuineyards in the Republic of Macedonia

  • Emilija Kostadinovska Goce Delcev University Faculty of Agriculture Department of Plant Protection and Environment
  • Sasa Mitrev Goce Delcev University Faculty of Agriculture Department of Plant Protection and Environment
  • Ilija Karov Goce Delcev University Faculty of Agriculture Department of Plant Protection and Environment
  • Violeta Dimovska Goce Delcev University Faculty of Agriculture

Abstract

Plant viruses are group of pathogens that cause and important loses in grapevine production and they have and great economic importance. They are obligate parasite form and for their replication they used plant cells.

In particular vines, the harmful effects of viral infection manifests itself in reduced yield and quality of grapes, reducing the life of the vines in grapevine calem production - reducing the yield and quality of cuttings for grafting and weak adhesion of the coupling place.

By applying serological ELISA technique and RT-PCR molecular diagnostics, we determining the presence of most distributed phloemic viruses in grapevine - Grapevine leafroll associated virus GlRaV (-1,-2,-3,-7). The study covered  total of 382 isolates of grapevine viruses, in the period from 2008 till 2014.

In relation to grapevine viruses, we monitored the presence of the leafroll virus in grapevine (grapevine leafroll associated virus) with groups -1 and -3 of this virus (GlRaV-1 and GlRaV-3).

References

Borgo M., Angelini E. (2002). Influence de l’enroulmentfoliare GLRaV-3 sur les parameters de la production du Merlot. Bullerin de I’O.I.V., 859-860.

Cabaleiro C., Seruga A. (1996). Efecto del enrollado de la vid (GLRaV-3) enunvinedoen plena produccion del cultivar “Albarino”. Investigation Agraria, Produccion y Proteccion Vegetal 11, 451-463.

Foissac X., Svanella-Dumas L., Gentit P., DulucgMJ.,Candresse T. (2000). Polivalent detection of fruit tree tricho, capillo and foveaviruses by nested RT-PCR using degenerated and inosine containing primers (PRO RT-PCR). 16th International Symposium of Virus and Virus-Like diseases on fruit trees. Canterbury p.48. ActaAgricultura, Vol 550, 37-43.

Hewit W.B. (1970). Virus and Virus diseases of the Grapevine. Review of Applied Mycology 47, 433-455.

International Committee on Taxonomy of Viruses. (2011). Virus taxonomy. http://www.ictvonline.org/virusTaxonomy.asp?version=2012

Mackenzie D. J., McLean M.A., Mukerji S., Green M. (1997). Imptoval RNA extraction from woody plants for the detection of viral pathogens by reverse transcription-polymerase chain reaction. Plant Disease 81. 222-226.

Martelli G.P., Agranovsky A.A., Bar-Joseph M., Boscia D., Candresse T., Coutts R.H.A., Dolja V.V., Falk B.W., Gonsalves D., Jelkmann W., Karasev A.V., Minafra A., Namba S., Vetten H.J., Wisler G.C. Yoshikawa N. (2002a). The family Closteroviridae revised. Archives of Virology 147, 2039-2043.

Martelli, G.P., Boudon-Padieu, E. (2006). Directory of Infectious Diseases of Grapevines and Viroses and Virus-like Diseases of the Grapevine: Bibliographic Report 1998-2004. Options Méditerranéennes, Série B: N.55, pp 297

Нацрт-стратегија за лозарство и винарство, 2010-2015

Peake B.K., Mackie A.E., Sivasithamparam K., Habili N., Mckirdy S.J. (2004). First report of grapevineleafroll associated virus 9 (GLRaV-9) in Western Australia. Australasian Plant Pathology, 33: 445–446.

Rowhani A., Uyemoto J.K. (1997): A comparision between serological and biological assay in detecting grapevine leafroll associated viruses. Plant Disease 81, 799-801.

Sforza R., Boudon-Padieu E., Greif C. (2003). New Mealybug Species Vectoring Grapevine Leafroll-Associated Viruses-1 and -3 (GLRaV-1 and -3). European Journal of Plant Pathology, Vol 109, 975-981.

Turturo C., Rott M. E., Minafra A., Saldarelli P., Jelkmann W., Martelli G. P. (2000).Partial molecular characterization and RT-PCR detection of Grapevine leafrollassociated virus 7. Extended abstracts 13th Meeting of ICVG, Adelaide, Australia, 17-18.

Voncina D. (2011). Utvrdjivanje virusa na autohtonim sortama vinove loze (Vitis vinifera L.) u Dalmaciji seroloskim, molekularnim i bioloskim metodama. Sveuciliste u Zagrebu, Agronomski fakultet (doktorska disertacija).

Published
2016-02-05