The Effect of Incubation Treatmen on the Pepper (Capsicum annuum L.) Androgenesis
Abstract
Genetics and breeders have a lot of troubles in obtaining of haploid plants. The number of plant species in which haploids in vivo spontaneous occurs is only bigger than 100, but this rule in the nature is very seldom. At the pepper the only type of explants, which formed somatic embryos are anthers, immature zygotic embryos and the callus formed from immature zygotic embryos. Androgenesis is new and assured method for creation of haploid plants, where the vegetative or generative nucleus from the pollen grain has been stimulated for developing in the haploid individual, without further fertilization. The purpose of this examination was to test the different incubation treatments in the anther culture in vitro of different pepper varieties.References
Collin H. A. i Edwards S. (1998): Plant Cell Culture, BIOS Scientific Publishers Limited, Oxford, UK.
Dolcet-Sanjuan R., Claveria, E., Huerta, A. (1997): Androgenesis in Capsicum annuum L. – Effects of Carbohydrate and Carbon Dioxide enrichment, J. Amer. Soc. Sci. 122(4):468-475.
Dumas de Valux, R., Chambonnet, D., Pochard, E. (1981): In vitro culture of pepper (Capsicum annuum L.) Anthers: high rate plant production from different genotypes by + 35ºC treatments. Agronomie 1(10): 859-864.
George L., and Narayanaswany, S. (1973): Haploid Capsicum through experimental androgenesis, Protoplasma 78, 467-470.
Matsubara, S., Hu, K. i Murakami, K. (1992): Embryoid and callus formation from pollen greins of eggplant and pepper by anther culture. Journal of the Society of Hortycultural Science 61: 69-77.
Mityko, J. (1996): Anthere Culture in pepper (Capsicum annuum L.), Agricultural Biotechnology Center, Gödöllõ, Hungary, Labaratory manual 1-8.
Mityko, J., Andrasfalvy, G., Csillery, G., Fary. M. (1995): Anther culture response in different genotypes and F1 hybrids of pepper (Capsicum annuum L.), Plant Breeding 114, 78-80.
Munyon, I.P., Hubstenberg, J.F. i Phillips, G.C.: (1989): Oring of plantlets and callus obtained from chile pepper anther cultures. In vitro Cellular and Developmental Biology 25:293-296.
Murashige, T., i Skoog, F. (1962): A revised medium for rapid growth and bio assay with tobacco tissue cultures, Phisiologya Planatarum, Vol. 15: 473-497.
Pierik R.L.M. (1998): In vitro Culture of Higher Plants, Department of Horticulture, Wageningen Agricultural University, The Netherland.
