Presence of Stolbur Phytoplasma on local variety Stanusina
Phytoplasmic changes as well as virus diseases in one of the most promising crops in the Republic of Macedonia, the grapevine, are among the least studied and researched pathogenic changes in the country. In contrast, in other European countries, these changes have been investigated very intensively with results in publications relevant to the sciences and practice.
Variety Stanusina (grapevine for red wine production), is autochthone variety From Tikves grapevine region, and we can still find there. For scientific investigation of the presence of phytoplasmas on Stanusina, this variety, opposite from the Chardonnay and Vranec, is less sensitive. The symptoms in the field caused by phytoplasma disease on the variety Stanusina, are opposite from the literature, yellowing rolling leaves, characteristic of white variety.
During the period dedicated to this research (2012-2013), the situation was constantly monitored from the aspect of symptomatology. The main aim of the study was from collected material analyzed in the Laboratory of plant and environment protection, to make typification of the present pathogens by using modern molecular methods of PCR / RFLP, by studying four phytoplasmic gene loci: 16S rRNA, tuf, vmp1 gene (stol - 1H0), stamp gene, for the detection of the type of the present phytoplasmas. We identify presence of phytoplasmas XII-A group in the Tikves grape region.
Key words: phytoplasmas, 16S rRNA, tuf, vmp1 (stol - 1H0), stamp