ASSESSMENT OF GENETIC DIVERSITY AMONG BARLEY VARIETIES WITH DIFFERENT ORIGIN USING SIMPLE SEQUENCE REPEAT (SSR) MARKERS

Abstract

Barley (Hordeum vulgare L.) is one of the principal cereal crops in the world, because it’s used as raw material in beer production, animal feed and human consumption. The propose of this research was to determine the genetic diversity among barley genotypes developed in different counties using the SSR marker. As an experimental material were used twenty-one two row barley varieties with different origin. Three varieties and two promising lines were Macedonian, two varieties were Serbian, two varieties had Croatian origin and the other twelve varieties were developed in Bulgaria. Eighteen SSR markers were used to characterize the genetic diversity among the barley varieties and two of them had high polymorphisms (MGB402 and MGB318). The calculated polymorphism information content (PIC) values range from 0.163 to 0.574, which shows the importance of the markers for future diversity analysis of barley. Primer MGB318 showed the highest PIC and for MGB391 was obtained the lowest PIC. Using Unweight Pair Group Method, dendrogram tree was constructed and all studied genotypes were divided in three main groups, branched into sun-subgroup. These results can be useful for barley germplasm management and design of new crosses for future breeding propose.

Key words: barley, simple sequence repeat, genetic diversity, polymorphism information content, varieties