Seed Production From Pepper Obtained in In Vitro Anther Culture
Abstract
The aim of these examinations was establishment of effective in vitrotechnology for study of haploid and diploid plant regenerants; induction ofembryogenesis in pepper anther culture; development of the embryos intoregenerants as well as successful adaptation and acclimatization of regenerantsfrom sterile to greenhouse conditions. Induction of somatic embryogenesis inanther culture in 12 from 19 exanimate genotypes of pepper was achieved.After successful acclimatisation of the regenerants under greenhouseconditions seed material from four pepper genotypes was collected: Piran,Kurtovska Kapija Sr, Zlaten Medal Sr and Féherözön. The collected seedmaterial is excellent possibility for further breeding processes, cytogenetics andother molecular level research.References
Dumas de Valux, R., Chambonnet, D., Pochard, E. (1981): In vitro culture of pepper (Capsicum annuum L.) Anthers: high rate plant production from different genotypes by + 35ºC treatments. Agronomie 1(10): 859-864.
George, L., Narayanaswamy, S., 1973. Haploid capsicum through experimental androgenesis. Protoplasma 78: 467–470.
Kaparakis, G. (1999): In vitro culture of pepper (Capsicum annuun L.), PhD theses - Kaparakis Georgis, Aristotle Univ. Hellas, submitted Univ. Nottingham, UK.
Koleva-Gudeva, Liljana (2003): Kultura na anteri od piperka (Capsicum annuum L.), Godisen zbornik na JNU Institut za juzni zemjodelski kulturi, God 3: 95-102.
Koleva-Gudeva, Liljana, Spasenoski, M., Trajkova Fidanka (2007): Somatic embryogenesis in pepper anther culture: The effect of incubation treatments and different media. Scientia Horticulturae 111:114-119
Kuo, J.S., Wang, Z.Z., Chien, N.F., Ku, S.J., Kung, M.L., Hsu, H.C., 1973. Investigation of the anther culture in vitro of Nicotiana and Capsicum annuum L. Acta Bot. Sin. 15 (1), 43–47.
Mityko, Judit, and Fari, M., (1997): Problems and results of doubled haploid plant production in pepper (Capsicun annuum L.) via anther and microspore culture, Hort. Biotech and breeding, ISHS 1997, Acta Hort. 447: 281-287.
Mityko, Judit, Andrasfalvy, G., Csillery, G., Fary, M. (1995): Anther culture response in different genotypes and F1 hybrids of pepper (Capsicum annuum L.), Plant Breeding 114, 78-80.
Mityko, Judit (1993): Obtention of Anther derived homozygous plants in pepper (Capsicum annuum L.), OMFB, Hungary No. 93-97-44-0468.
Wang, J.J., Sun, C.S., Wang, C.C., Chein, N.F., 1973. The induction of pollen plantlets of Triticale and Capsicum annuum from anther culture. Sci. Sinica XVI (1), 147–151.
